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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lipase-Producing Microorganisms for Use in Contaminated Fat and Oil Kitchen Wastewater Treatment) ผู้เขียน: นางอรพิน ภูมิภมร, รองศาสตราจารย์ , ดร.เกรียงไกร พัทยากร, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractBiodegradation of fats and oils in wastewater has a potential role in pollution control. Then, selection of efficient microorganisms for degrading fats and oils in laboratory level has been investigated by using single culture of Acinetobacter sp. (KUL8), Bacillus sp. (KUL39) and Pseudomonas sp. (KLB1) and mixed cultures of Acinetobacter sp. (KUL8) and Pseudomonas sp. (KLB1). The result showed that single culture of Pseudomonas sp. (KLB1) and mixed cultures of Acinetobacter sp. (KUL8), Bacillus sp. (KUL39) and Pseudomonas sp. (KLB1) could be reduced fat and oil content by 55.91% and 60.42%, respectively. These cultures were shown highest efficiency activities. Thus, these cultures were selected to study speed of shaking and inoculum size in wastewater treatment, which it was found that the efficiency of fat and COD degradation were highest reduced at 250 rpm of speed of shaking. Inoculum size at 1-2.5 % and 5 % of single culture and mixed cultures could remove fat and oil by 61.78-65.19 % and 60.42 %, respectively. And initial COD concentration at 4,000 mg/l has the highest efficiency 83.46% and 95.81% in fat and COD degradation, respectively. The last experiment, Pseudomonas sp. (KLB1) was used in occasionally influent and effluent contaminated fat and oil kitchen wastewater treatment in reactor. The results showed that the highest efficiency of aerobe conditions were 50.02% and 0.76 U/ml, respectively in fat degradation and lipase activity after adding 8% of single culture at 22 days. After that lipase activity was reduced because influent and effluent of wastewater in reactor depended on activity of kitchen. Since dilution rates (D) of wastewater in reactor were higher than specific growth rate (?), so microorganisms were washed out. Thus, the immobilization of microorganism cells on plastic balls has been used. The results showed that cells immobilization could increase efficiency of wastewater treatment in reactor. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Optimum use of Salinity, Nitrate and Pond Depth for b-Carotene Production of Dunaliella salina) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, นายอุดม สิทธิภู่ประเสริฐ, อาจารย์,  Naiyana Boontaveeyuwat, ดร.จันทนา ไพรบูรณ์, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractDunaliella salina, halotorelant green algae was collected from the East Coast of Thailand. It has a massive accumulation of b-carotene when grown under defined growth conditions such as high light intensity, high salt concentration and nitrate deficiency. The present study investigated the optimization of salinity, nitrate and pond depth for cell growth and b-carotene production of the alga in question. Cultivation was done in three stages. These were indoor cell growth cultivation, outdoor cell growth cultivation and outdoor b-carotene production. The optimum salinity for cell growth of indoor and outdoor cultivation (5 l) was 9% NaCl, which has specific growth rate (m) of 0.579 (d-1) and 0.981 (d-1). The optimum salinity for outdoors b-carotene production (5 l) when use 40% inoculum (2.16 ? 106 cell ml-1) was 12% NaCl. This produced b-carotene content of 51.73 mg ml-1. In addition, the concentrations of medium used were 100%, 75% and 50% to decrease nitrate concentration in the starter ponds for increasing b-carotene production in the next step. The results showed that 50% medium gave minimum nitrate concentration of 4.5 mg l-1. For the effect of pond depth, the light expose of the alga were used 9, 11, 13 and 18 cm the ratios of carotenoid to chlorophyll were 7.48, 6.25, 5.54 and 3.35, respectively. Therefore the suitable pond depth for b-carotene production from D. salina was 9 to 11 cm. |
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 การประชุมวิชาการProduction and composition of sulfated polysaccharides as physiological active substance from marine algae of Thailandผู้แต่ง:Dr.Anong Chirapart, Professor, Dr.Kangsadan Boonprab, Associate Professor , J. Praiboon, O. Bhumibhamon, J. Phothikasikorn, Yoshihiko Akakabe, Kenji Matsui, Tadahiko Kajiwara, การประชุมวิชาการ: |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isolation and Characterization of Pseudomonas sp. KLB1 Lipase from High Fat Wastewater) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Jantana Jinda, Suptawee Fungthong สื่อสิ่งพิมพ์:pdf AbstractSix isolates of lipase-producing micro-organisms were screened from two types of wastewater. The bacterial isolates were KLB1, KLB2, KLB3 and isolated yeasts were KLY1, KLY2 and KLY3. The isolate showed highest lipase activity was KLB1 which later was identified as Pseudomonas sp. Lipase of Pseudomonas sp. KLB1 was found to be an inducible enzyme with palmolein. The model for lipase production was growth associate pattern. As regards to the physicochemical properties, the Pseudomonas sp. KLB1 lipase had maximal activity at 50?C and pH 9. For its stability, even though this enzyme showed the maximal stability at pH 7.0 and 37?C, its stability was increased when incubated at pH 8.0-10 and 37, 50, 60, and 70?C. The residue activity was 76% and 76.23% at pH 10, 70?C and pH 9, 37?C. However, the lipase showed two pH stability ranges that possible indicated two types of lipases were formed in Pseudomonas sp. KLB1. The lipase was activated by Ca2+, K+,and Na+, (NH4)2S2O3 and ascorbic acid but inhibited by Zn2+, Mn2+, Co2+, KI and EDTA. The enzyme is also more specific on the medium and long chain triacylglycerol of vegetable oil than of animal fat. The Km and Vmax of tributyrin hydrolysis were 110.9 mM, and 2.45 mM s-1, respectively whereas these kinetic parameters from palmplein hydrolysis were 1,188.8 mM, and 5.25 mM s-1, respectively. |
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หัวเรื่อง:การหมักโกโก้ III: การปรับปรุงการหมักโกโก้โดยเสริมการหมักด้วยกล้าเชื้อผสมในระดับห้องปฏิบัติการและเกษตรกร ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Jantana Jinda, บุญให้ แหลมเพชร, Piyanut Naka สื่อสิ่งพิมพ์:pdf AbstractIn studied improvement of cocoa fermentation by inoculated with selected culture in laboratoryscale the microorganisms used were 3 strains of alcohol-producing yeasts, pectinase-producing yeasts and bacteria, and 6 strains of acetic acid bacteria. After cocoa fermentation in 5 kg batches of each strains, the strains which showed better results in fully fermented (4/4F) cocoa by cut-test method were Saccharomyces cerevisiae (KU-Y77), Saccharomyces chevalieri (KU-Y150) and Acetobacter aceti (KU-A72). The optimum ratio of these strains in 20 kg cocoa batches were 1.5:1.0:1.0 (% weight) which given fully fermented cocoa in 81.7-83.5% where as 43.7-61.0% were found in natural fermentation batches. Four farm-trials cocoa fermentation experiment with inoculated mix-culture were carried on 4 and 6 days. The results showed fully fermented cocoa in 79-84% which was indicated as cocoa FAO grade 1 standard. In the same time, the cocoa fermentation period was able to reduce from 6 days to 4 days. Anyhow, the utilization of inoculum in small scale (20-40 kg) showed promising. However, the quality of mix-culture fermented cocoa should be further investigated by sensory evaluation method. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Properties of Starch and Protein Isolated from Certain Mungbean Lines) ผู้เขียน:ดร.อรอนงค์ นัยวิกุล, ศาสตราจารย์ , นางจิตธนา แจ่มเมฆ, ผู้ช่วยศาสตราจารย์, นางอรพิน ภูมิภมร, รองศาสตราจารย์, Woatthaichai Narkrugsa สื่อสิ่งพิมพ์:pdf AbstractTwenty mungbean lines were analysed for chemical compositions. The results showed that they contanined 7 to 9% moisture, 19 to 24% protein and 59 to 65% carbohydrates. Starch and protein were isolated from three mungbean lines, VC 2742, VC 2745, VC 2755 and the cultivar U – thong 1, using 0.1N.Na2 Co3 solution. The amount of isolated startches were 12 to 22%, having the purity of 82 to 89% while the amount of isolated proteins were 12 to 17%, having the purity of 70 to 77%. Using scanning electron microscope to examine the mungbean flour and starch granules, the results showed that the flour had many components attached to starch granules while the isolated starch showed rather clear starch granule, circle, oblong or kidney-like shapes with the size of 14 to 19 microns in width and 21 to 29 microns in length. The functional properties of isolated starch and protein were determined. The results showed that starch isolated from the three mungbean lines had 84 to 93% water absorption, 72 to 80% oil absorption and minimum gelation at 16 to 20% starch by volume which were not quite difference from that of U-Thong 1,. The isolated protein had much lower amount of water absorption, oil absorption and minimum gelation than isolated starch from the same source and some-what difference from U-Thong 1. Protein isolated from U-Thong 1 had less water and oil absorption, but required almost twice protein to form gel, compared to the protein isolated from the three mungbean lines. |
ที่มา:Kasetsart Journal: Natural Sciencesหัวเรื่อง:Bhumibhamon, Orapin; Jinda, Jantana; Fungthong, Suptawee. Isolation and characterization of Pseudomonas sp. KLB1 lipase from high fat wastewater. |
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